Coding

Part:BBa_K3581000:Design

Designed by: HUI Wan Hei   Group: iGEM20_HKUST   (2020-10-13)


Hagfish intermediate filaments alpha subunit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Addition of Acc65I and AscI cut sites in front and after the coding region for insertion of chromoproteins.


Source

Thread biopolymer filament subunit alpha from Eptatretus stoutii (Pacific hagfish).

References

1. Jing, F., A. Guerette, P., Pavesi, A., Horbelt, N., Chwee Teck, L., J. Harrington, M., & Miserez, A. (2017). Artificial hagfish protein fibers with ultra-high and tunable stiffness, 9(35), 12908–12915. The Royal Society of Chemistry.